Journal: Biomarker Insights
Article Title: The Therapeutic Potential of Blocking Galectin-3 Expression in Acute Myocardial Infarction and Mitigating Inflammation of Infarct Region: A Clinical Outcome-Based Translational Study
doi: 10.1177/1177271918771969
Figure Lengend Snippet: Post-MI myocardial galectin-3 expression and macrophage infiltration 1 week after LAD ligation. Wild-type mice showed scattered areas of galectin-3 positivity (Panel A1), which was absent in the myocardial sections obtained from galectin-3 KO mice (Panel A2). Panel A3 represents isotype control for galectin-3 antibody. Panel A4 represents quantitative analysis of Panels A1 and A2 (7 myocardial fields, each group). The macrophage-specific F4/80 staining in WT mice showed abundant positively stained cells in the infarct (red arrow) and peri-infarct (blue arrow) regions, especially in the areas of excess cardiomyocyte loss (Panel B1). The galectin-3 KO mice showed reduced F4/80 positive cellular infiltration compared with WT mice (Panel B2). Panel B3 represents isotype control for mouse F4/80 antibody. Panel B4 represents quantitative analysis of Panels B1 and B2 (12 myocardial fields, each group). KO indicates knock-out; LAD, left anterior descending; MI, myocardial infarction; WT, wild-type. * P < .05.
Article Snippet: To measure serum galectin-3, a pre-coated galectin-3 specific 96-well strip microplate Enzyme-linked Immunosorbent Assays (ELISA) kit (R&D Systems; Minneapolis, MN, USA) was used, and optical densities were analyzed with standard galectin-3 concentrations (at ng/mL) based on the manufacturer’s recommendations.
Techniques: Expressing, Ligation, Staining, Knock-Out